High-Density Detection of Restriction-Site-Associated DNA Markers for Rapid Mapping of Mutated Loci in Neurospora
نویسندگان
چکیده
منابع مشابه
High-density detection of restriction-site-associated DNA markers for rapid mapping of mutated loci in Neurospora.
The wealth of sequence information available for Neurospora crassa and other fungi has greatly facilitated evolutionary and molecular analyses of this group. Although "reverse" genetics, in which genes are first identified by their sequence rather than by their mutant phenotypes, serves as a valuable new approach for elucidating biological processes, classical "forward" genetic analysis is stil...
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Upland cotton (Gossypium hirsutum L., 2n = 52, AADD) is an allotetraploid, therefore the discovery of single nucleotide polymorphism (SNP) markers is difficult. The recent emergence of genome complexity reduction technologies based on the next-generation sequencing (NGS) platform has greatly expedited SNP discovery in crops with highly repetitive and complex genomes. Here we applied restriction...
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Genetic maps in barley are usually constructed from a limited number of molecular markers such as SSR (simple sequence repeat) and DarT (diversity arrays technology). These markers must be first developed before being used for genotyping. Here, we introduce a new strategy based on sequencing progeny of a doubled haploid population from Baudin × AC Metcalfe to construct a genetic map in barley. ...
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Salinity stress is one of the most widespread soil problems next to drought, in rice growing areas. ReducingSodium (Na+), while maintaining Potassium (K+) uptake in rice are traits that would aid in salinity tolerance.Therefore, the identification of quantitative trait loci (QTLs) associated with those for Na+ and K+uptake, will enable breeders to use marker-assisted selection...
متن کاملAlgorithms for the restriction-site mapping of DNA molecules.
Two algorithms are described that rapidly construct restriction maps of DNA molecules from single- and double-digest data. The implementation of these algorithms into the Pascal programs AMAPK and AMARD allows such mapping to be done on microcomputers.
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ژورنال
عنوان ژورنال: Genetics
سال: 2007
ISSN: 1943-2631
DOI: 10.1534/genetics.107.078147